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Current issue: Volume 24, No. 2 (2024)
DOI: https://doi.org/10.59893/abud.24(2).002
Korotka I., Volkogon V. 2024. The process of nitrification in the root zone of winter rye plants under the action of mineral nitrogen and inoculation. Acta Biol. Univ. Daugav., 24(2): 165-176.
Abstract
Among the most important components of the nitrogen cycle in nature, the nitrification process is the least studied. Furthermore, nitrification features in soils depend on many factors, the role of some of which is almost unknown. The inhibitory approach to determining the nitrification activity allows for a differential assessment of the participation of auto- and heterotrophic nitrifiers in the oxidative link of the nitrogen cycle. Our study was conducted in a field experiment on cultivated sod-podzolic dusty-sandy soil. Winter rye was grown without inoculation and with Diazobacteryn treatment on different backgrounds. The experiment determined the content of water-soluble protein with Folin reactive and the activity of nitrate reductase in the flag leaf, protein content of grain, carried out yield accounting. The intensity of nitrification increases with the application of mineral nitrogen fertilisers in the experiment. When fertilizers were applied at a dose of N120, the activity of the process in the phase of tube emergence increased by 38%, flowering − by 54%, and milk ripeness − by 93% compared to the control (without fertilizers). We found that pre-sowing inoculation of winter rye seeds with a biological product based on Azospirillum brasilense, stimulates the activity of autotrophic and heterotrophic nitrification, which may be one of the factors of positive influence of bacteria introduced into the agrocenosis on the root nutrition of plants. Autotrophic nitrification significantly exceeds the activity of the heterotrophic process. At the same time, the activity of autotrophic nitrification relative contribution to the formation of the nitrate pool in the rhizosphere soil of plants increases within the growing season. The use of Diazobacteryn helps to intensify the activity of heterotrophic nitrifiers, especially in the early stages of plant vegetation.
Keywords: Autotrophic nitrifiers, heterotrophic nitrifiers, inoculation, winter rye, nitrification inhibitor.
*Corresponding author: Iryna Korotka. Institute of Agricultural Microbiology and Agroindustrial Manufacture NAAS, Department of Economics, Intellectual Property, Marketing of Innovations and Organisation of Scientific Personnel Training, Shevchenko Str., 97, Chernihiv, 14027, Ukraine. E-mail: chychvaga_i@i.ua. ORCID: https://orcid. org/0000-0002-2864-4049 Vitalii Volkogon. Institute of Agricultural Microbiology and Agroindustrial Manufacture NAAS, Department of Soil Microbiology, Shevchenko Str., 97, 14027, Chernihiv, Ukraine. ORCID: https://orcid.org/0000-0003-0675-1318
