Effectiveness of doubled haploids production by anther culture from selected winter wheat hybrids

Current issue: Volume 20, No. 2 (2020)

EFFECTIVENESS OF DOUBLED HAPLOIDSPRODUCTION BY ANTHER CULTURE FROM SELECTED WINTER WHEAT HYBRIDS

Kārlis Žagata, Dace Grauda, Isaak Rashal

Žagata K., Grauda D., Rashal I. 2020. Effectiveness of doubled haploids production by anther
culture from selected winter wheat hybrids. Acta Biol. Univ. Daugavp., 20 (2): 107 – 111.

Abstract

Using of doubled haploids (DH) allows shorten the crop breeding process by 3–4 years. Anther culture is one of the most effective methods of obtaining DH lines of different crops including wheat: doubling chromosome set of haploid plants, grown in vitro from pollen, resulting in homozygous plants. The objective of this work was obtaining DH lines by anther culture from winter wheat hybrids. For evaluation of embryos formation efficiency 60 different winter wheat hybrids were examined. Among them five winter wheat hybrids with the highest embryogenesis level were chosen for obtaining DH lines. From 8460 anthers placed on the induction medium 2113 embryos were obtained, which finally resulted in 505 plants-regenerants. Genotype effect on plants-regenerants production was observed. Obtained plants-regenerants were passed to Stende Research Centre of the Institute of Agricultural Resources and Economics for testing from the aspect of breeding.

Key words: DH lines, plants-regenerants, genotype effect.

Kārlis Žagata, Dace Grauda, Isaak Rashal. Institute of Biology, University of Latvia, 1 Jelgavas Street, Rīga, LV-1004, Latvia, E-mail: karlis.zagata@lu.lv, dace.grauda@lu.lv, izaks.rasals@lu.lv