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Current issue: Volume 20, No. 2 (2020)
TESTING THE MICROSATELLITES-PCR MARKERS FOR GENETIC DIVERSITY RESEARCH OF ALIEN PONTOCASPIAN AMPHIPOD PONTOGAMMARUS ROBUSTOIDES G. O. SARS, 1894
Aija Brakovska, Jana Paidere
Abstract
Brakovska A., Paidere J. 2020. Testing the microsatellites-PCR markers for genetic diversity research of alien Ponto-Caspian Amphipod Pontogammarus robustoides G. O. Sars, 1894. Acta Biol. Univ. Daugavp., 20 (2): 95 – 105.
Microsatellites are successfully used for genetic studies for the monitoring of different Gammarus species. But microsatellites markers for the study of Pontogammarus robustoides population are not enough developed. Since the development of specific microsatellites primers requires time and material investments, therefore it may be used for research the primers designed to related species. We tested six developed microsatellites markers Dv-6, Dv-11, Gam-1, Gam-2, Gapu-9 and Gapu-17 for five related Gammarus species (Gammarus pulex, Gammarus fossarum, Gammarus roeselii, Gammarus orinos, Dikerogammarus villosus), which showed the applicability of these markers for these species (Danancher et al. 2009, Gergs et al. 2010, Rewicz et al. 2014). Microsatellites-PCR based molecular markers are powerful tools for the analysis of genetic diversity of Pontogammarus robustoides specimens for which isolation of good quality genomic DNA and suitable microsatellites-PCR marker and protocol development is essential. This paper present a comparison of qualitative DNA isolation method and microsatellite-PCR markers for adults specimens of Pontogammarus robustoides population genetic study.
Key words: alien Ponto-Caspian amphipod, Pontogammarus robustoides, microsatellites-PCR.
Aija Brakovska, Jana Paidere. Institute of Life Sciences and Technology, Department of Ecology, Daugavpils University, Parādes str.1a, LV-5401, Daugavpils, Latvia, E-mail: aija.brakovska@inbox.lv
